Alexey Tomilin

Professor, Head of Laboratory of Cellular and Molecular Biology

Laboratory of Cellular and Molecular Biology

Alexey Tomilin, Ph.D., D.Sc., Prof.
Corresponding Member of the Russian Academy of Sciences
Date/place of birth: 25.03.1970, Leningrad
Head of Laboratory,
Institute of Cytology,
Russian Academy of Science,
Tikhoretsky avenue 4,
194064 St-Petersburg,
tel: +7-(812)-297-4519
Institute of Translational Biomedicine,
St-Petersburg University,
Universitetskaya nab. 7-9,
199034 St-Petersburg,
tel: +7 (812) 3636939


IstinaResearcherID (IRID): 51743332
ResearcherID: C-3361-2014
1987-1992    Master Degree, Physics School, Polytechnical University, St-Petersburg, Russia.
1992-1997    Post-graduate training, Institute of Cytology, Russian Academy of Sciences (RAS), St-Petersburg, Russia and University of Caen, France; Ph.Ds. (Russian and French);
1998:            EMBO post-doctoral fellowship (1998);
1998-1999    post-doctoral training, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany; advisor Dr. Hans R. Scholer.
1999-2002    post-doctoral training, School of Veterinary Medicine, University of Pennsylvania, USA; advisor Dr. Hans R. Scholer.
2002-2006    Junior group leader, Dept. Developmental Biology, Max-Planck Institute for Immunobiology, Freiburg, Germany
2007-now     Lab leader, Institute of Cytology RAS, St-Petersburg, Russia;
2009             D.Sc. degree;
2011             Elected Corresponding Member of the Russian Academy of Science;
2009-now     Member of scientific and thesis defence board of the Institute of Cytology RAS;
2009-2016    Advisory Board member of the Int. Rev. Cell Mol. Biol.;
2011             Elected corresponding member of the RAS;
2013             Member of the progmam committee of the FEBS-2013;
2013-now     Member of the Expert Board of the Russian Foundation of Basic Research;
2014-now     Member of Expert Board of the Russian Science Foundation;
2014             Expert of the Presidium of the RAS programme "Fundamental research for the development of biomedical technologies";
2014-now     Member of Scientific Council of the Ministry of Health;
2016             Сhair of the conference “Recent Achievements in Stem Cells Research” (CTERP 2016), April 6-8, 2016, St. Petersburg, Russia;
2016             Member of selection committee of the 12-th International Congress of Cell Biology (ICCB 2016), Praha;
2016             Honorary medal "Silver Sigma" (Siberian Branch of the Russian Acad. Sci.)
2017-2018    Co-Сhair of the conference “Recent Achievements in Stem Cells Research” (CTERP 2018), April 11-13, 2018, Moscow, Russia;
2017             Honorary Diploma of the Russian Academy of Sciences "For conscientious work, great contribution to the development of fundamental and applied science, in connection with the 60th anniversary of the foundation of the Institute of Cytology";
2017             Member of program committee of the III National Congress of Regenerative Medicine, November 15-18, 2018, Moscow, Russia;
2008-2011       Mikhail Liskovykh (currently a post-doc at the National Cancer Institute, NIH, Bethesda)
2011-2016       Tatyana Starkova
2012-2016       Alexander Khudyakov
2013-now         Sergey Ponomartsev
2014-now         Evgeny Bakhmet
2015-now         Andrey Kuzmin
1. A method of using stem cell in tissue replacement therapy (Tomilin A., Tolkunova E., Liskovykh M.) Patent of the Russian Federation No.2422514, 2011.

2. A method of to achieve a safety of using pluripotent stem cells in tissue replacement therapy with the help of artificial chromosomes (Tomilin A., Liskovykh M.), Patent of the Russian Federation №2548819, 2015.

  1. Bakhmet E.I.Nazarov I.B.Artamonova T.O.Khodorkovsky M.A.Tomilin A.N. (2017) Mass spectrometry for identification of proteins that specifically bind to a distal enhancer of the Oct4 gene. Journal of Physics: Conference Series 917: 1-5.
  2. Kulichkova V.A., Artamonova T.O., Lyublinskaya O.G., Khodorkovskii M.A., Tomilin A.N.Tsimokha A.S. (2017) Proteomic analysis of affinity-purified extracellular proteasomes reveals exclusively 20S complexes. Oncotarget 8(60): 102134-102149.
  3. Khudiakov A, Kostina D, Zlotina A, Yany N, Sergushichev A, Pervunina T, Tomilin A, Kostareva A, Malashicheva A. (2017) Generation of iPSC line from patient with arrhythmogenic right ventricular cardiomyopathy carrying mutations in PKP2 gene. Stem Cell Res. 2017 Oct;24:85-88. doi: 10.1016/j.scr.2017.08.014.
  4. Khudiakov A, Kostina D, Zlotina A, Nikulina T, Sergushichev A, Gudkova A, Tomilin A, Malashicheva A, Kostareva A. (2017) Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene. Stem Cell Res. 24:77-80. doi: 10.1016/j.scr.2017.08.015.
  5. Bajenova O., Tolkunova E., Koshkin S., Malov S., Thomas P., Tomilin A., O’Brien S. (2017) The Role of the Carcinoembryonic Antigen Receptor in Colorectal Cancer Progression. J Integr Oncol 2017, 6: 2. DOI: 10.4172/2329-6771.1000192
  6. Selenina A.B., Tsimokha A.S., Tomilin A.N. (2017) Proteasomes in regulation of protein homeostasis of pluripotent stem cells. Acta Naturae 9(3): 39-47.
  7. Starkova T., Polyanichko A., Artamonova T., Khodorkovskii, M., Kostyleva E., Chikhirzina E., Tomilin A. (2017) Post-translational modifications of linker histone H1 variants in mammals. Phys. Biol. 14(1): 016005,
  8. Koshkin S, Danilova A, Raskin G, Petrov N, Bajenova O, O'Brien SJ, Tomilin A, Tolkunova E. (2016) Primary cultures of human colon cancer as a model to study cancer stem cells. Tumor Biol. 2016 Jul 23. DOI: 10.1007/s13277-016-5214-8
  9. Cherepanova OA , Gomez D, Shankman LS, Swiatlowska P, Williams O, Sarmento OF, Alencar GF, Hess DL, Bevard MH, Greene ES, Murgai M, Turner SD, Geng YJ, Bekiranov S, Connelly JJ, Tomilin A., Owens GK (2016) Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nat Medicine  22(6): 657-662. doi:10.1038/nm.4109

  10. Kostina AS, Uspensky VЕ, Irtyuga OB, Ignatieva EV, Freylikhman O, Gavriliuk ND, Moiseeva OM, Zhuk S, Tomilin A, Kostareva АА, Malashicheva AB (2016) Notch-dependent EMT is attenuated in patients with aortic aneurysm and bicuspid aortic valve. Biochim Biophys Acta. 2016 Feb 10. pii: S0925-4439(16)30032-1. doi: 10.1016/j.bbadis.2016.02.006.
  11. Khudiakov A.A., Kostina D.A., Kostareva A.A., Tomilin A.N., Malashicheva A.B. (2016) The effect of plakophilin-2 gene mutations on the activity of canonical WNT signaling. Cell Tiss. Biol.  10: 106. (translation of Tsitilogia/Цитология). DOI: 10.1134/S1990519X16020061
  12. Nazarov I, Chekliarova I, Rychkov G, Ilatovskiy AV, Crane-Robinson C, Tomilin A.  (2016) AFM studies in diverse ionic environments of nucleosomes reconstituted on the 601 positioning sequence. Biochimie 121: 5-12. DOI: 10.1016/j.biochi.2015.11.010
  13. Liskovykh M, Ponomartsev S, Popova E, Bader M, Kouprina N, Larionov V, Alenina N, Tomilin A. (2015) Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice. Cell Cycle 4(8):1268-73. IF=4.56. DOI: 10.1080/15384101.2015.1014151.
  14. Davydov-Sinitsyn, A.P., Bajenova, O.V., Liskovykh, M.A., S. V. Ponomartsev, I. V. Rykov, S. A. Koshkin, R. V. Orlova, A. N. Tomilin, E. N. Tolkunova (2015) Tumor growth suppression after xenografting of human colorectal carcinoma cells. Cell Tiss. Biol. 9: 318. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X15040045
  15. Kulichkova VA, Artamonova TO, Zaykova JJ, Ermolaeva JB, Khodorkovskii MA, Barlev NA, Tomilin AN, Tsimokha AS. (2015) Simultaneous EGFP and Tag Labeling of the b7 Subunit for Live Imaging and Affinity Purification of Functional Human Proteasomes. Mol. Biotechnol. 57(1): 36-44. DOI: 10.1007/s12033-014-9799-0
  16. Kouprina N, Tomilin A, Masumoto H, Earnshaw WC, Larionov V. (2014) HAC-based gene delivery vectors for gene function studies, gene therapy and pharmacology. Expert Opin. Drug Del. 11(4): 1-19. DOI: 10.1517/17425247.2014.882314
  17. Kulichkova, V.A., Zaykova, Y.Y., Ermolaeva, Y.B., E. V. Vinogradova, L. Huang, A. N. Tomilin, N. A. Barlev, A. S. Tsimokha (2014) Creation of a HEK293 cell line stably expressing the proteasome subunit PSMD14 fused with fluorescent protein EGFP and HTBT tag. Cell Tiss. Biol. 8: 330. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X14040051
  18. Nazarov, I.B., Krasnoborova, V.A., Mitenberg, A.G., E. V. Chikhirzhina, , A. P. Davidov-Sinitzin, M. A. Liskovykh, A. N. Tomilin (2014) Transcription regulation of Oct4 (Pou5F1) gene by its distal enhancer. Cell Tiss. Biol. 8: 27. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X14010106
  19. Schneider, T A; Fishman, V S; Liskovykh, M A; Ponamartsev, S V; Serov, O L; Tomilin, A N; Alenina, N. Reprogramming of somatic cells. Problems and solutions. Tsitologiia 56(12): 869-80. (in Russian). PubMed ID:25929128.
  20. DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra PW, Nagy A, van der Kooy D (2013) Oct4 is required for e7.5 for proliferation in the primitive streak. PLoS Genet. 9(11): e1003957. DOI: 10.1371/journal.pgen.1003957
  21. Wu G, Han D, Gong Y, Sebastiano V, Gentile L, Singhal N, Adachi K, Fischedick G, Ortmeier C, Sinn M, Radstaak M, Tomilin A, Schöler HR. (2013) Establishment of totipotency does not depend on Oct4. Nat Cell Biol 15(9):1089-97. DOI: 10.1038/ncb2816
  22. Lee NC, Kononenko AV, Lee HS, Tolkunova EN, Liskovykh MA, Masumoto H, Earnshaw WC, Tomilin A, Larionov V, Kouprina N. (2013) Protecting a transgene expression from the HAC-based vector by different chromatin insulators. Cell Mol Life Sci. 70(19): 3723-37. DOI: 10.1007/s00018-013-1362-9.
  23. Davydov-Sinitsyn, A.P., Bajenova, O.V., Liskovykh, M.A. , S. V. Ponomartsev, L. L. Chechik, A. N. Tomilin, E. N. Tolkunova (2013) Comparative analysis of colorectal carcinoma cell lines that differ in metastatic potential. Cell Tiss. Biol. 7: 407. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X13050040
  24. Davydov-Sinitsyn A. P., O. V. Bazhenova, M. A. Liskovykh, L. L. Chechik, S. V. Ponomartsev, A. N. Tomilin, E. N. Tolkunova (2013) In vitro derivation and characterization of a colorectal cancer stem cell subpopulation.Cell Tiss. Biol 7(4): 320-324. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X13040044
  25. Liskovykh M., Kouprina N., Larionov V., Tomilin A. (2012) Artificial chromosomes for gene therapy and tissue replacement. Cell Transpl. and Tissue Engeneering VII(4), 6-18.
  26. Liskovykh M., Chuykin I., Ranjan A., Safina D., Tolkunova E., Minina J., Zhdanova N., Dyban P.A., Mullins J.J., Kostyleva E.I., Chikhirzhina E.V., Bader M., Alenina N., Tomilin A. (2012) Generation of rat-induced pluripotent stem cells: Reprogramming and culture medium. Cell Tiss. Biol 6(2): 115-121. doi:10.1134/S1990519X12020095
  27. Liskovykh M., Chuykin I., Ranjan A., Popova E., Tolkunova E., Chechik L., Mosienko V., Bader M., Alenina N., Tomilin A.  (2012) Genetic manipulations and examination of differentiation properties of rat induced pluripotent cells. Cell and Tissue Biol. 6(2):122-127. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X12020101
  28. Liskovykh, M.A., Davydov-Sinitcyn, A.P., Marilovtceva, E.V., A. N. Tomilin, E. N. Tolkunova (2012) Interaction between the CDX2 transcription factor and DDX5 protein. Cell Tiss. Biol. 6: 20. (translation of Tsitilogia/Цитология). doi:10.1134/S1990519X12010051
  29. Tomilin A.N. (2012)  On The 2012 Nobel Prize in Physiology or Medicine. Acta Naturae  4(4): 8-10.
  30. Liskovykh M., Chuykin I., Ranjan A., Safina D., Popova E., Tolkunova E., Mosienko V., Minina Iu., Zhdanova N., Mullins J.J., Michael Bader M., Alenina N., Tomilin A. (2011) Derivation, Characterization, and Stable Transfection of Induced Pluripotent Stem Cells from Fischer344 Rats. PLoS One 11(6): e27345.
  31. Minina Y., Zhdanova N., Shilov A., Tolkunova E., Liskovykh M. and Tomilin A. (2010) Chromosomal Instability of Mouse Pluripotent Cells Cultured in Vitro. Cell and Tissue Biol. 4(3): 223-227.
  32. Kuckenberg P., Buhl S., Woynecki T., van Fürden B., Tolkunova E., Seiffe F., Moser M., Tomilin A., Winterhager E., Schorle H. (2010) The transcription factor TCFAP2C/AP-2gamma cooperates with CDX2 to maintain trophectoderm formation. Mol. Cell. Biol. 30: 3310-3320.
  33. Saxe J., Tomilin А., Schöler H.R., Plath K., Huang J. (2009) Post-Translational Regulation of Oct4 Transcriptional Activity. PLoS One 4: 1-9.
  34. Tolkunova E., Malashicheva A., Chikhirzhina E., Kostyleva E., Zeng W., Luo J., Dobrinski I., Hierholzer A., Kemler R., and Tomilin A. (2009) E-Cadherin as a Novel Surface Marker of Spermatogonial Stem Cells. Cell and Tissue Biol. 3(2): 103-109.
  35. Popov B., Petrov N., Mikhailov V., Tomilin A., Alekseenko L., Grinchuk T., and Zaichik A. (2009) Spontaneous Transformation and Immortalization of Mesenchymal Stem Cells in vitro. Cell and Tissue Biol. 3(2): 110-120.
  36. Tolkunova A., Malashicheva A., Parfenov V.N., Sustmann C., Grosschedl R., Tomilin A. (2007) PIAS proteins as repressors of Oct4 function. J. Mol. Biol. 374: 1200-1212.
  37. Lengner C.J., Camargo F.D., Hochedlinger K., Welstead G.G., Zaidi S., Gokhale S., Schöler H.R., Tomilin A., Jaenisch R. (2007) Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1: 403–415.
  38. Malashicheva A., Kanzler B., Tolkunova E., Trono D., Tomilin A. (2007) Lentivirus as a tool for lineage-specific gene manipulations. Genesis 45, 456-459.
  39. Tolkunova E., Cavaleri F., Eckardt S., Reinbold R., Christenson L.K.,  Schöler H.R. , Tomilin A. (2006) The Caudal-Related Protein Cdx2 Promotes Trophoblast Differentiation of Mouse ES Cells. Stem Cells 24: 139-144.
  40. Kehler J., Tolkunova E., Koschorz B., Pesce M., Gentile L., Boiani M., Lomeli H., Nagy A., McLaughlin K.J., Schöler H.R., Tomilin A. (2004) Oct4 is required for primordial germ cell survival. EMBO Rep. 5:1078-1083.
  41. Lins K., Reményi A., Tomilin A., Massa S., Wilmanns M., Matthias P. and Schöler H.R. (2003) OBF1 enhances transcriptional potential of Oct1. EMBO J. 22: 2188-2198.
  42. Seralini GE, Tomilin A, Auvray P, Nativelle-Serpentini C, Sourdaine P, Moslemi S. (2003) Molecular characterization and expression of equine testicular cytochrome P450 aromatase. Biochim Biophys Acta. 1625: 229-238.
  43. Remenyi A, Tomilin A, Schöler HR, Wilmanns M. (2002) Differential activity by DNA-induced quarternary structures of POU transcription factors. Biochem Pharmacol. 64: 979-984.
  44. *Remenyi A., *Tomilin A., Pohl E., Lins K., Philippsen A., Reinbold R., Schöler H.R., and Wilmanns M. (2001) Differential transcriptional activity of dimeric Oct-1 by DNA-motif induced domain swapping. Mol. Cell 8: 569-580. *Equal contributors.
  45. *Tomilin A., *Remenyi A., Lins K., Bak H., Leidel S., Vriend G., Wilmanns M., Scholer H.R. (2000) Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Cell 103: 853-864. *Equal contributors.
  46. Kropotov A., Sedova V., Ivanov V., Sazeeva N., Tomilin A., Krutilina R., Oei S.L., Griesenbeck J., Buchlow G., Tomilin N. (1999) A novel human DNA-binding protein with sequence similarity to a subfamily of redox proteins which is able to repress RNA-polymerase-III-driven transcription of the Alu-family retroposons in vitro. Eur. J. Biochem. 260(2): 336-346.
  47. Tomilin A., Vorob'ev V., Drosdowsky M., Seralini G.-E. (1998) Oct3/4-associating proteins from embryonal carcinoma and spermatogenic cells of mouse. Mol. Biol. Rep. 25(2): 103-109.
  48. Belokopytova I.A., Kostyleva E.I., Tomilin A., Vorob'ev V.I. (1993) Human male infertility may be due to a decrease of the protamine P2 content in sperm chromatin. Mol. Reprod. Dev. 34(1): 53-57.